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  • Research use only (RUO). All products are sold strictly for laboratory and research purposes — not for human or veterinary consumption. Purchasers must be 21 or older.

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    NAD+

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    Select strength and vial count — prices shown for the selected SKU

    Buy the 10-pack — save 47%/mg

    2 single vials minimum for standalone shipping — or add samples to any pack

    Lab-direct quality — full packs or single-vial samples

    Every batch ships straight from the lab that synthesises it — sealed, tamper-evident, and HPLC-verified to >99% purity . Buying direct means you pay the lab-direct rate on every vial, with nothing stacked on top.

    Order a full sealed 10-vial research pack for a complete study supply, or add a single-vial sample alongside your pack to trial a new compound first. Same lab, same batch, same verified purity — scaled to whatever your research needs.

    What It's Researched For

    In plain terms, NAD+ is the molecule cells use to move energy around and to switch repair machinery on. Here is what that looks like across the research.

    Mitochondrial function

    Studied as the carrier that hands electrons to the mitochondria — the step that makes ATP, the cell’s energy currency.

    Cellular-ageing models

    Tissue NAD+ falls with age in model organisms, so restoring it is one of the most-tested ideas in longevity biology.

    DNA-repair signalling

    PARP enzymes burn through NAD+ when they detect DNA damage, linking genome maintenance directly to metabolic supply.

    Circadian biology

    NAD+ levels oscillate on a daily cycle and feed back onto the clock machinery through SIRT1 — a standard readout in chronobiology.

    Overview

    In short: NAD+ is the cell’s central redox coenzyme and the fuel for the sirtuin, PARP and CD38 signalling enzymes — the single molecule that links energy metabolism to DNA repair, gene regulation and cellular ageing.

    Key research facts

  • The central redox coenzyme of metabolism — cycles between NAD+ and NADH to carry electrons through glycolysis, the TCA cycle and β-oxidation
  • Delivers reducing equivalents to complex I of the electron-transport chain, the entry point of mitochondrial ATP production
  • Obligatory substrate for the sirtuin deacylases (SIRT1–SIRT7) studied in metabolic and cellular-ageing models
  • Required co-substrate for PARP1/PARP2 during DNA-damage detection and repair signalling
  • Cleaved by CD38 / CD157 to generate cyclic ADP-ribose, a calcium-mobilising second messenger
  • Research use only. These points summarise lab themes, not human outcomes.

    Nicotinamide adenine dinucleotide is a dinucleotide: an adenine nucleotide and a nicotinamide nucleotide joined through a pair of phosphates. That nicotinamide ring is the working end. It accepts a hydride ion to become NADH and gives it back to become NAD+ again, and this one reversible reaction underpins glycolysis, the tricarboxylic-acid cycle, fatty-acid oxidation and — via complex I of the electron-transport chain — the bulk of mitochondrial ATP production. In this role NAD+ is a catalyst: it is recycled, not used up.

    The second role is what makes it interesting to ageing research. Three enzyme families cleave NAD+ and consume it outright. The sirtuins (SIRT1–SIRT7) use it to remove acetyl and other acyl groups from histones and metabolic enzymes, coupling gene regulation to metabolic state. The poly(ADP-ribose) polymerases use it to build ADP-ribose chains at sites of DNA damage. CD38 and CD157 hydrolyse it into cyclic ADP-ribose, a calcium-mobilising second messenger. Because these reactions destroy the molecule, the cell must constantly rebuild it — mostly through the salvage pathway, where the enzyme NAMPT recycles nicotinamide, and to a lesser extent from the dietary precursors nicotinamide riboside and nicotinamide mononucleotide or de novo from tryptophan.

    Those two roles collide with age. NAD+ concentrations fall measurably in the tissues of ageing model organisms, at the same time as CD38 expression rises and PARP activity increases with accumulated DNA damage — more demand, less supply. Whether raising NAD+ changes any downstream outcome in humans remains an open research question rather than a settled result. It is supplied here as a research-grade lyophilised powder for in-vitro and preclinical use only, and nothing on this page is medical advice.

    Mechanism of Action

    NAD+ works two ways at once: as a recycled redox carrier shuttling electrons into the mitochondrial electron-transport chain, and as a consumed substrate for the sirtuin, PARP and CD38 enzyme families that couple metabolic state to gene regulation, DNA repair and calcium signalling.

    Pathway Effect Why it matters NAD+/NADH redox couple Accepts and donates a hydride ion across glycolysis, the TCA cycle and β-oxidation The core electron-carrying reaction of intermediary metabolism Complex I / oxidative phosphorylation NADH delivers reducing equivalents to complex I of the electron-transport chain Entry point for the proton gradient that drives ATP synthesis Sirtuin deacylation (SIRT1–SIRT7) Consumed as co-substrate to strip acetyl/acyl groups from histones and metabolic enzymes Direct molecular link between NAD+ availability and gene regulation PARP1 / PARP2 signalling Consumed to build poly(ADP-ribose) chains at sites of DNA strand breaks Couples genome maintenance to the cell’s metabolic budget CD38 / CD157 hydrolysis Cleaves NAD+ into cyclic ADP-ribose and nicotinamide Generates calcium-mobilising second messengers; a major age-related NAD+ sink NAMPT salvage pathway Rate-limiting recycling of nicotinamide back into NAD+ The main resupply route and the usual target of NAD+ research interventions Deeper dive for scientific readers

    The cell keeps NAD+ and NADH in a strongly oxidised ratio — free cytosolic NAD+:NADH is typically several hundred to one — because that gradient is what makes the redox reactions of glycolysis thermodynamically favourable. Compartmentalisation matters: cytosolic, mitochondrial and nuclear pools are regulated separately, and the inner mitochondrial membrane is impermeable to intact NAD+, so shuttles (malate–aspartate, glycerol-3-phosphate) rather than direct transport move reducing equivalents between them. This compartmentalisation is why the route and form of NAD+ used in an experiment change the result. On the consumption side, the picture that has emerged from ageing models is one of competing sinks: PARP activity climbs as DNA damage accumulates, CD38 expression rises with inflammatory ageing, and both draw down the same pool that sirtuins depend on — the mechanistic basis for the widely studied hypothesis that sirtuin activity falls in ageing tissue because its substrate does. Exogenous NAD+ itself is a large, charged molecule with poor cell permeability and is substantially degraded extracellularly by CD38 and the ectoenzyme CD73, which is why much of the field works with the smaller precursors NR and NMN and why route, infusion rate and formulation dominate the experimental literature on intact NAD+.

    Common Questions People Are Asking

    What is NAD+ used for in research?

    NAD+ is studied as the central redox coenzyme of metabolism and as the consumed substrate of the sirtuin, PARP and CD38 enzyme families. Research examines mitochondrial electron transport and ATP production, sirtuin-mediated gene regulation, PARP-dependent DNA-repair signalling, calcium second-messenger generation, circadian oscillation of the NAD+ pool, and the age-related decline of tissue NAD+ in cellular-senescence models. It is supplied here for laboratory research only and is not for human use.

    What is the difference between NAD+ and NADH?

    They are the two halves of one redox couple. NAD+ is the oxidised form; it accepts a hydride ion (two electrons and a proton) to become NADH, the reduced form, which then donates that hydride elsewhere — most importantly to complex I of the mitochondrial electron-transport chain — regenerating NAD+. Cells hold the free cytosolic pool strongly toward NAD+, and that ratio is what makes the oxidative steps of glycolysis thermodynamically favourable.

    How does NAD+ differ from NMN and nicotinamide riboside?

    NMN and NR are precursors — smaller molecules that cells convert into NAD+ through the salvage pathway. NAD+ is the finished coenzyme. The practical difference is delivery: NAD+ is large and doubly charged, so it crosses membranes poorly and is broken down extracellularly by CD38 and CD73, which is why most oral human research uses NR or NMN while intact NAD+ research uses parenteral routes.

    Why does NAD+ decline with age?

    Model-organism data point to both sides of the ledger changing at once. Supply falls as NAMPT salvage-pathway activity decreases, while demand rises: PARP enzymes consume more NAD+ as DNA damage accumulates, and CD38 expression increases with inflammatory ageing. Since sirtuins draw on the same pool, the widely studied hypothesis is that sirtuin activity falls in ageing tissue because its substrate does.

    How should NAD+ be stored?

    Keep the lyophilised powder frozen at −20 °C, protected from light and moisture. After reconstitution in bacteriostatic water or sterile saline, refrigerate at 1–6 °C and use promptly — NAD+ hydrolyses in solution, and degradation accelerates at alkaline pH and at room temperature. Aliquot after reconstitution and avoid repeated freeze-thaw cycles where the protocol allows.

    Where can I buy NAD+?

    Right here — NAD+ is supplied directly by New-U Research Compounds on this page. Every batch is independently third-party tested to >99% HPLC purity with a batch-linked Certificate of Analysis, supplied as lyophilised research-grade material, and shipped direct from source worldwide in discreet, tracked packaging. Strictly for laboratory research use only — not for human use.

    How much does NAD+ cost?

    NAD+ pricing is shown live on this page, per pack size — 10-vial research packs as standard, with single-vial sample options on selected compounds. Larger vial strengths lower the per-mg cost, every order includes the batch Certificate of Analysis, and shipping is free on orders over $300.

    Is NAD+ third-party tested?

    Yes. Every NAD+ batch is verified by independent laboratories (Janoshik Analytics and Freedom Diagnostics) for identity and purity, with a batch-linked Certificate of Analysis confirming >99% purity by HPLC. Every order ships with its COA, and current batch certificates are published on our COA page.

    How do I buy NAD+?

    Add the NAD+ pack size you need to your cart and check out: enter your shipping details, then choose your payment method — cryptocurrency or card — on the next step. Every order ships with its batch Certificate of Analysis (COA). NAD+ is supplied strictly for laboratory research use only, not for human or veterinary use.

    What payment methods can I use to buy NAD+?

    At checkout you can pay by cryptocurrency (BTC, ETH, SOL, LTC, USDC, USDT and more) or by card, each handled by a dedicated secure payment provider. You choose your method after confirming your order.

    How fast is shipping, and do you ship worldwide?

    Yes — we ship worldwide in discreet, unmarked, temperature-stable, tracked packaging. Delivery typically takes 6–14 business days, and shipping is free on orders over $300.

    Is it legal to buy NAD+?

    In the United States, NAD+ is sold strictly for laboratory and research purposes only. It is not approved by the FDA for human consumption and is not sold for that purpose. Regulatory status varies by jurisdiction — buyers are responsible for compliance in their own region.

    Pharmacokinetics

    Half-life Short in circulation — intact extracellular NAD+ is rapidly degraded by CD38 and ectonucleotidases such as CD73 Absorption route Poor across the gut wall and across intact cell membranes; the molecule is large and doubly charged at physiological pH Bioavailability Oral NAD+ is largely hydrolysed to nicotinamide and nicotinamide riboside before absorption; parenteral routes dominate the research literature Metabolism / clearance Degraded extracellularly to nicotinamide mononucleotide and nicotinamide riboside, then salvaged intracellularly back to NAD+ via NAMPT/NMNAT Stability Hydrolytically unstable in solution, degrading faster at alkaline pH and at ambient temperature; the lyophilised powder is stable frozen Notes Published research protocols describe slow intravenous infusion over several hours because rapid administration is poorly tolerated in the reported literature. These are descriptions of published experimental practice, not guidance for use.

    Evidence Tier

    Overall: Mixed (human + animal)

    The biochemistry of NAD+ is textbook-established: its redox role, its consumption by sirtuins, PARPs and CD38, and the NAMPT salvage pathway are among the best-characterised facts in metabolism, and the age-related decline of tissue NAD+ is reproduced across multiple model organisms. What is not established is the interventional claim. Human trials have largely studied the precursors nicotinamide riboside and nicotinamide mononucleotide rather than intact NAD+, and while they report reliable increases in blood NAD+ metabolites, functional outcomes remain inconsistent. Interventional data on intravenous NAD+ itself is limited to small, mostly uncontrolled studies. As a supplied research compound it is unapproved laboratory material, not an approved drug.

    Tier 1 · Human clinical

  • Human trials of the NAD+ precursors nicotinamide riboside and nicotinamide mononucleotide report raised blood NAD+ metabolites with inconsistent functional endpoints; no trial citation for intact intravenous NAD+ is provided on this page
  • Tier 2 · Animal

  • Age-related decline of tissue NAD+, and restoration of metabolic and mitochondrial readouts by precursor administration, are reproduced across rodent and invertebrate models
  • Certificates, databases & peer-reviewed sources

    Last reviewed: 5 August 2026 · New-U Research Compounds

  • PubMed: peer-reviewed literature on NAD+
  • ClinicalTrials.gov: registered studies on NAD+
  • NAD+: Wikipedia
  • WebMD: consumer health reference
  • BBC News: Health
  • CNN Health: “Peptides: what to know about the wellness trend”
  • Sky News: “Can peptides make America healthy again?”
  • Sky News: “Inside the exploding US peptides craze” (video)
  • Sky News Australia: “Black market peptide trade explodes as influencers fuel uptick in use”
  • Sky News Australia: “Backyard peptide boom sparks alarm” (video)
  • Sky News Australia: “Oprah reveals struggle with shame of weight-loss drugs”
  • Key Characteristics

  • Dinucleotide coenzyme, not a peptide — adenine and nicotinamide nucleotides bridged by two phosphates
  • Molecular formula C₂₁H₂₇N₇O₁₄P₂; molecular weight 663.43 Da (free acid); CAS 53-84-9
  • Recycled as a redox carrier (NAD+ ⇌ NADH) but consumed by sirtuins, PARPs and CD38
  • Resupplied mainly through the NAMPT salvage pathway from nicotinamide
  • Cytosolic, mitochondrial and nuclear pools are separately regulated
  • Large and doubly charged — poor membrane permeability, poor oral bioavailability
  • Hydrolytically unstable in solution; store the lyophilised powder frozen
  • Research-grade purity: >99% HPLC — confirm against the supplied batch certificate
  • Specifications

    Molecular Formula C 21 H 27 N 7 O 14 P 2 Molecular Weight 663.43 Da (free acid) CAS Number 53-84-9 Purity >99% (HPLC) Form Lyophilised powder Class Endogenous dinucleotide redox coenzyme Route Intravenous infusion or subcutaneous (research protocols) Solubility Bacteriostatic water or sterile saline; freely water-soluble Storage Lyophilised: −20 °C freezer. Reconstituted: 1-6 °C, away from light; hydrolyses in solution.

    About NAD+ (Nicotinamide Adenine Dinucleotide): Redox Coenzyme and Sirtuin Substrate Research Guide

    NAD+ is one of the few molecules that turns up in every chapter of a metabolism textbook. It is a dinucleotide — an adenine nucleotide joined to a nicotinamide nucleotide through a pyrophosphate bridge — and its nicotinamide ring can accept a hydride ion to become NADH and release it again to become NAD+. That single reversible step is the electron-carrying backbone of glycolysis, the tricarboxylic-acid cycle and fatty-acid oxidation, and it is how reducing equivalents reach complex I of the mitochondrial electron-transport chain, where the proton gradient that drives ATP synthesis begins.

    What moved NAD+ from textbook biochemistry to the centre of ageing research is its second, non-catalytic role. The sirtuins — SIRT1 through SIRT7 — cannot deacylate histones or metabolic enzymes without consuming NAD+, which makes them direct sensors of the cell’s metabolic state. The poly(ADP-ribose) polymerases consume it to mark DNA strand breaks. CD38 and CD157 hydrolyse it into cyclic ADP-ribose for calcium signalling. All three destroy the molecule, so the cell continuously rebuilds it, chiefly through the NAMPT-driven salvage of nicotinamide and secondarily from the precursors nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN) or de novo from tryptophan. In ageing tissue, supply falls while PARP and CD38 demand rises — the mechanistic core of the NAD+ decline hypothesis.

    The honest position on the interventional question is that it is unsettled. Human studies have overwhelmingly used the oral precursors NR and NMN rather than intact NAD+, and although they reliably raise circulating NAD+ metabolites, functional endpoints have been inconsistent across trials. Data on intravenous NAD+ itself is limited to small and largely uncontrolled work. New-U Research Compounds supplies NAD+ as a research-grade lyophilised powder at >99% HPLC purity, strictly for in-vitro and preclinical research use. It is not approved for human therapeutic use in any jurisdiction, and nothing on this page constitutes medical advice or a dosing recommendation.

  • Nicotinamide adenine dinucleotide - Wikipedia
  • Sirtuin - Wikipedia
  • Poly (ADP-ribose) polymerase - Wikipedia
  • CD38 - Wikipedia
  • Nicotinamide mononucleotide - Wikipedia
  • Nicotinamide riboside - Wikipedia
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    Descriptive catalog comparison for research sourcing decisions — not dosing guidance. All compounds are for laboratory research use only.

    More on NAD+

  • NAD+ research guide
  • Research use only — not for human consumption. All products are supplied strictly for laboratory research purposes.

    © 2026 New-U Research Compounds · new-u.io — Copyright held with Hilxera Distribution Services LLC. All rights reserved.