Bacteriostatic water is USP-grade sterile water containing 0.9% benzyl alcohol as a preservative - the standard solvent used to reconstitute lyophilised research peptides into injectable solutions.
Almost every peptide on this site is supplied as a lyophilised (freeze-dried) powder. Lyophilisation removes water from the peptide while preserving its structure, which dramatically extends shelf life - but before the peptide can actually be injected or used in a research protocol, you need to reconstitute it. Bacteriostatic water is the standard solvent for that job.
The "bacteriostatic" part refers to the 0.9% benzyl alcohol added as a preservative. Benzyl alcohol does not kill bacteria outright (which would make the water bactericidal), but it inhibits their growth strongly enough that a multi-dose vial stays sterile for up to 28 days at 2-8 °C once opened. That is the practical advantage over plain sterile water, which would need to be used immediately after opening.
Bacteriostatic water is the minimum required accessory for any peptide research protocol. New-U Research Compounds supplies it as USP-grade, tamper-evident vials for reconstituting lyophilised research peptides.
Bacteriostatic water is sterile water for injection with 0.9% benzyl alcohol - a preservative that inhibits bacterial growth without killing the bacteria outright.
| Pathway | Effect | Why it matters |
|---|---|---|
| Benzyl alcohol preservative | 0.9% v/v inhibits bacterial replication | Keeps the vial usable for multi-dose research protocols over 28 days |
| USP grade sterility | Manufactured and filtered to USP pharmaceutical standards | Safe for injection use in research protocols that require sterile solvents |
| Peptide compatibility | Neutral pH, preservative non-reactive with most peptides | Standard solvent of choice across essentially all research peptide work |
| Dissolution | Dissolves lyophilised peptide cakes gently | Restores the peptide to an injectable or usable solution form |
| Multi-dose shelf life | 28 days once opened when refrigerated | Supports typical research dosing protocols without requiring a new solvent vial for every dose |
Bacteriostatic water for injection is a USP compendial article defined as sterile water containing 0.9% w/v benzyl alcohol. The preservative concentration is deliberately chosen to inhibit microbial growth in multi-dose vials without exceeding the safe benzyl alcohol exposure threshold for typical dosing volumes. Note that benzyl alcohol is contraindicated for neonates and is not recommended for dilution in very large volumes, which is a standard clinical caution carried over into research handling.
Both are sterile at the point of manufacture, but bacteriostatic water contains 0.9% benzyl alcohol as a preservative that inhibits bacterial growth after the vial is opened. Plain sterile water for injection is single-use: once you puncture the seal, it should be used immediately. Bacteriostatic water can safely be drawn from multiple times over 28 days if refrigerated.
Typically 1-2 mL per vial, but the exact volume depends on the desired final concentration and how the peptide will be dosed. For example, reconstituting a 10 mg vial in 2 mL gives 5 mg/mL, so 0.1 mL = 0.5 mg. Always calculate in advance based on the protocol you are following.
To avoid a direct stream of water hitting the lyophilised peptide cake. A harsh stream can shear the peptide and risk denaturation, and it also creates foam which traps peptide. Slow injection against the glass wall lets the water dissolve the cake gently - swirl afterwards rather than shaking.
No - freezing is not recommended. The benzyl alcohol preservative can separate from the water on freeze-thaw, and the product is designed to be stored at room temperature when sealed or at 2-8 °C once opened. Do not freeze, and do not use if the solution appears cloudy or particulate.
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| Stability | Room temperature when sealed; refrigerate at 2–8 °C once opened. Do not freeze. Discard 28 days after first use. |
|---|---|
| Notes | Draw desired volume with a sterile syringe, inject slowly into peptide vial at an angle against the glass wall. Swirl gently, do not shake. |
| Dose | Frequency | Duration | Notes |
|---|---|---|---|
| Use 1–2 ml per peptide vial depending on desired concentration | As needed for reconstitution | – | Draw desired volume with a sterile syringe, inject slowly into peptide vial at an angle against the glass wall. Swirl gently, do not shake. |
Dissolves lyophilised peptide powders into injectable or usable solutions - the essential first step of every research protocol.
Bacteriostatic water on Wikipedia →0.9% benzyl alcohol preservative inhibits bacterial growth for 28 days after first use when refrigerated.
Manufactured and sterilised to USP pharmaceutical compendial standards.
Sealed vials with tamper-evident closures for sterility assurance.
Compatible with essentially all research peptides as the standard reconstitution solvent.
Use 1-2 mL per peptide vial to achieve desired working concentration.
| Composition | Sterile water + 0.9% w/v benzyl alcohol (USP grade) |
|---|---|
| Use | Reconstitution solvent for lyophilised research peptides |
| Typical volume | 1-2 mL per peptide vial (depends on desired concentration) |
| Form | Sterile aqueous solution in sealed multi-dose vials |
| Shelf life (opened) | 28 days at 2-8 °C |
| Compatibility | Essentially all research peptides |
| Sterility | Sealed, tamper-evident vials |
| Preservative | Benzyl alcohol 0.9% |
| Storage | Room temperature sealed; 2-8 °C once opened |
Bacteriostatic water is the unglamorous but essential piece of equipment for any peptide research protocol. Virtually every peptide on this site is shipped as a lyophilised powder, which preserves the molecule's structure and dramatically extends shelf life. But before you can actually use the peptide, you need to reconstitute it in a sterile solvent - and bacteriostatic water is the standard solvent of choice for that job across the entire research peptide field.
The key feature is the 0.9% benzyl alcohol preservative. Plain sterile water for injection is single-use because any residual bacteria introduced during withdrawal can grow over time. Benzyl alcohol at 0.9% inhibits that growth strongly enough that a multi-dose vial remains sterile for 28 days at refrigerator temperatures, which matches the typical timeline for a single-vial peptide research protocol.
New-U Research Compounds supplies bacteriostatic water as USP-grade, sealed, tamper-evident vials designed for research peptide reconstitution. Proper handling matters: draw the desired volume with a sterile syringe, inject slowly against the inner wall of the peptide vial at an angle, and swirl gently - do not shake, as vigorous agitation can denature peptides. Nothing on this page is medical advice.